Table 1.

DNA methylation status in barley leaves and roots under specific water conditions

MSAP band type a	Leaves			Roots
	Control	Water deficit stress	Rewatering	Control	Water deficit stress	Rewatering
I	180	194	202	162	152	181
II	156	131	152	96	87	79
III	303	273	286	288	298	278
IV	8	49	7	7	16	15
Total analysed bands	647	647	647	553	553	553
Total methylated bandsb	467	453	445	391	401	372
Fully methylated bandsc	311	322	293	295	314	293
MSAP (%)d	72.1	70.0	68.8	70.7	72.5	67.3
Hemi-methylated ratio (%)e	24.1	20.3	23.5	17.4	15.7	14.3
Fully methylated ratio (%)f	48.1	49.8	45.3	53.4	56.8	53.0

^a Type I is the presence of bands in both EcoRI/HpaII and EcoRI/MspI digests and indicates the absence of methylation; type II are bands generated in EcoRI/HpaII digests but not in EcoRI/MspI digests; type III bands appear in EcoRI/MspI digests but not in EcoRI/HpaII digests; and type IV represents the absence of band in both enzyme combinations

^b Total methylated bands are II+III+IV bands.

^c Fully methylated are III+IV bands.

^d MSAP is a percentage ratio of total methylated bands (II+III+IV) to total amplified bands.

^e Hemi-methylated ratio is a percentage ratio of total hemi-methylated bands (II) to total amplified bands.

^f Fully methylated ratio is a percentage ratio of fully methylated bands (III+IV) to total amplified bands.