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. 2016 Feb 12;95(6):e2769. doi: 10.1097/MD.0000000000002769

FIGURE 2.

FIGURE 2

Seropositivity to E4, E7, and VLPs-L1 in serum of healthy women and women with CC by Slot blot system. In vitro purified HPV antigens were immobilized in PROTRAN membranes using the Hybri-Slot system, and membranes were fixed and blocked as described in “Materials and Methods.” Strips containing the 3 HPV16 antigens (E4 10 ng, E7 10 ng, VLPs-L1 200 ng) and negative controls (buffer alone and GFP 200 ng) were incubated with dilutions (1:2,500) of sera from women with CC (A), sera from healthy women (B), and positive and negative female serum controls for anti-HPV antibodies (C). Biotinylated secondary antibody (dil 1:10,000) and Streptavidin-horseradish peroxidase (1:8,000) were used to develop the system, and the bands were finally visualized by chemiluminescence in the Odyssey Fc system. CC = cervical cancer, GFP = green fluorescent protein, HPV = human papillomavirus, VLPs-L1 = virus-like particles from L1 protein.