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. 2015 Dec 31;25(5):878–891. doi: 10.1093/hmg/ddv617

Figure 2.

Figure 2.

Assessment of nucleus compaction and DNA breaks of sperm from OAF patients. (A) Histones content of sperm was assessed using the AB test. The histogram shows the percentage of stained sperm in samples from control (n = 5 ± SD) and patients (n = 1). (B) Protamination of sperm was evaluated using the chromomycin A3 test, and results are displayed in the histogram, which shows the percentage of stained sperm in control (n = 5 ± SD) and patients' (n = 1) samples. (C) The histogram of DNA fragmentation analysis with TUNEL assay showing the level of TUNEL-positive sperm in control (n = 5 ± SD) and patients' (n = 1) samples.