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. 2015 Dec 31;25(5):878–891. doi: 10.1093/hmg/ddv617

Figure 4.

Figure 4.

PLCZ1 expression is undetectable by IF and WB in sperm of patients exhibiting OAF after ICSI. (A1) Overlay of Hoechst staining (blue) and PLCZ1 staining (green) of sperm from a fertile control patient showing strong immunoreactivity in the post-acrosomal area (white arrow heads and inset) and to a lesser extent in the acrosome. (A2) Representative staining of sperm from patients with OAF showing the absence of PLCZ1 staining in the post-acrosomal area and very faint staining over the acrosome. More than 100 sperm were observed per patient. (B) WB using protein extracts of sperm from a fertile control (Ctl), or Patient 1 (P1) and Patient 2 (P2) and an anti-PLCZ1 antibody that fails to detect reactivity in the patients' lanes. (C) Protein gel representing loading control for Figure 1B showing that all lanes were similarly loaded. Protein loads were controlled with TGX stain free™ precast gels.