Figure 6.

Ile489Phe PLCZ1 induces fewer Ca²⁺ oscillations compromising embryo development. (A) Injection of hPLCZ1 cRNA (0.001 μg/μl) in MII mouse oocytes triggered high-frequency Ca²⁺ oscillations, and between 6 and 10 rises per hour that end by 120–180 min after injection. (B) In contrast, injection of cRNA of Ile489Phe hPLCZ1 (0.001 μg/μl) triggered oscillations that were either delayed and with a low frequency (17/31) or failed to initiate them (14/31). (C) Histogram showing means + SD of the frequency of Ca²⁺ rises in function of control or Ile489Phe cRNA PLCZ1 concentrations injected into oocytes; asterisks indicate significant differences determined by t-test, P < 0.01. (D) Rates of 2PN, two-cell embryos and blastocysts obtained after injection of WT or Ile489Phe cRNA PLCZ1 (0.01 μg/μl) in mouse MII oocytes; n corresponds to the number of injected oocytes.