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. 2016 Jan 6;25(5):936–950. doi: 10.1093/hmg/ddv627

Figure 2.

Figure 2.

Mutants of Hrb98DE and hnRNPA2 co-localize with stress granule marker ROX8 in cytoplasmic inclusions in Drosophila indirect flight muscle. (A) Day 2 adult Drosophila expressing ROX8-GFP (green) with either WT or D302V Flag-Hrb98DE (red) were dissected to examine the cellular localization of these proteins. Flag-Hrb98DE WT localized in nuclei, and Flag-Hrb98DE D302V co-localized with ROX8-GFP in cytoplasmic inclusions. Scale bar: 20 µm. (B) The MFI of Flag-Hrb98DE D302V in ROX8-positive foci was significantly higher than that of Flag-Hrb98DE WT. P < 0.0001, t-test. (C) The percentage of the fluorescence signal of Flag-Hrb98DE D302V co-localized with ROX8-GFP was significantly higher than that of WT. P < 0.01, t-test. (D) Day 2 adult Drosophila expressing GFP-ROX8 (green) with either WT or D290V hnRNPA2 (red) were dissected to examine the cellular localization of these proteins. hnRNPA2 WT localized in nuclei, and hnRNPA2 D290V co-localized with ROX8-GFP in cytoplasmic inclusions. Scale bar: 20 µm. (E) The MFI of hnRNPA2 D290V in ROX8-positive foci was significantly higher than that of hnRNPA2 WT. P < 0.05, t-test. (F) The percentage of the fluorescence signal of hnRNPA2 D290V co-localized with ROX8-GFP was significantly higher than that of WT. P < 0.01, t-test.