Figure 4.
Expression of MRJ inhibited Hrb98DE D302V-positive cytoplasmic inclusion formation and rescued abnormal wing posture phenotype. (A) Either WT or D302V Flag-Hrb98DE was co-expressed either with luciferase or with MRJ in Drosophila indirect flight muscle. The cellular localization of Flag-Hrb98DE was detected by anti-Flag antibody. (B) Cytoplasmic re-localization of Flag-Hrb98DE D302V was enhanced, following knockdown of MRJ levels using a deficiency allele. (C) The percentage of nuclear Flag-Hrb98DE D302V signals was significantly increased when it was co-expressed with MRJ (P < 0.0001, t-test). (D) Co-expression of MRJ rescued the abnormal wing posture phenotype caused by Flag-Hrb98DE D302V. P < 0.0001, χ2 test. Scale bar in (A) and (B), 20 µm.