TABLE I.
Sequence-specific primers for HLA-DRB1 alleles
| Allele | Primer sequences | PCR product size (bp) | |
|---|---|---|---|
|
| |||
| 5′-Sequence 5′→3′ | 3′-Sequence 5′→3′ | ||
| DRB1*03 | TACTTCCATAACCAGGAGGAGA | TGCAGTAGTTGTCCACCCG | 151 |
| DRB1*04 | GTTTCTTGGAGCAGGTTAAACA | CTGCACTGTGAAGCTCTCAC | 260 |
| DRB1*07 | CCTGTGGCAGGGTAAGTATA | CCCGTAGTTGTGTCTGCACAC | 232 |
| DRB1*08 | AGTACTCTACGGGTGAGTGTT | CTGCAGTAGGTGTCCACCAG | 214 |
| DRB1*09 | GTTTCTTGAAGCAGGATAAGTT | CCCGTAGTTGTGTCTGCACAC | 236 |
| DRB1*11 | GTTTCTTGGAGTACTCTACGTC | CTGGCTGTTCCAGTACTCCT | 176 |
| DRB1*12 | ACTCTACGGGTGAGTGTT | ACTGTGAAGCTCTCCACAG | 244 |
| DRB1*13 | TACTTCCATAACCAGGAGAGA | CCCGCTCGTCTTCCAGGAT | 130 |
| DRB1*14 | GTTTCTTGCAGTACTCTACGTC | TCTGCAATAGGTGTCCACCT | 224 |
| DRB1*15 | TCCTGTGGCAGCCTAAGAG | CCGCGCCTGCTCCAGGAT | 197 |
| DRB1*16 | TCCTGTGGCAGCCTAAGAG | CTCCGTCACCGCCCGGT | 137 |
| HGF | CAGTGCCTTCCCAACCATTCCCTTA | ATCCACTCACGGATTTCTGTTGTGTTTC | 432 |
PCR = polymerase chain reaction.