TABLE II.
Specific polymerase chain reaction system for HLA-DRB1 alleles
| Allele | Premix Taq (μL) | Reference (μL) | Primer (μL) | ddH2O (μL) | DNA (μL) | ||
|---|---|---|---|---|---|---|---|
|
|
|
||||||
| 5′-SSP | 3 ′-SSP | 5 ′-SSP | 3 ′-SSP | ||||
| DRB1*03 | 12.5 | 0.5 | 0.5 | 0.3 | 0.3 | 8.9 | 2 |
| DRB1*04 | 12.5 | 0.5 | 0.5 | 0.3 | 0.3 | 8.9 | 2 |
| DRB1*07 | 12.5 | 0.4 | 0.4 | 0.5 | 0.5 | 8.2 | 2 |
| DRB1*08 | 12.5 | 0.6 | 0.6 | 0.2 | 0.2 | 8.9 | 2 |
| DRB1*09 | 12.5 | 0.4 | 0.4 | 0.5 | 0.5 | 8.2 | 2 |
| DRB1*11 | 12.5 | 0.3 | 0.3 | 0.4 | 0.4 | 9.1 | 2 |
| DRB1*12 | 12.5 | 0.3 | 0.3 | 0.5 | 0.5 | 8.9 | 2 |
| DRB1*13 | 12.5 | 0.2 | 0.2 | 0.7 | 0.7 | 9.7 | 1 |
| DRB1*14 | 12.5 | 0.3 | 0.3 | 0.4 | 0.4 | 9.1 | 2 |
| DRB1*15 | 12.5 | 0.5 | 0.5 | 0.5 | 0.5 | 8.5 | 2 |
| DRB1*16 | 12.5 | 0.6 | 0.6 | 0.2 | 0.2 | 8.9 | 2 |
SSP = single specific primer; ddH2O = double-distilled water.