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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Biochem J. 2015 Oct 5;472(3):309–318. doi: 10.1042/BJ20150019

Figure 4. Overexpression of PAMM inhibited the MAPK signalling pathway.

Figure 4

Raw264.7 cells were transiently transfected with PAMM expression plasmid or control plasmid. After 24 h, the transfected cells were incubated overnight, then stimulated with 100 ng/ml LPS for 0, 15 and 60 min as indicated. Cell lysates were extracted and Western blot was performed to detect the phosphorylation of ERK1/2 (A), p38 (B) and pJNK (C) with specific antibodies as indicated. Total protein levels of ERK1/2, p38 and JNK were also detected to serve as loading controls. Three independent experiments showed similar results.