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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Biomaterials. 2016 Jan 6;83:257–268. doi: 10.1016/j.biomaterials.2016.01.021

Figure 3. LDL-DiR Uptake into the Brain after FUS: Fluorescence Imaging.

Figure 3

Xenogen IVIS optical imaging was used to track the DiR fluorescence in the brain. A. Whole brain (top) and cross-section (bottom) of brain from rats injected intravenous with LDL-DiR in presence or absence of FUS. B. Fluorescence Microscopy. Digital fluorescent image from coronal cryosection of the brain. Representative areas of interest are expanded from FUS exposed region (left) and contralateral hemisphere (right). Images were captured at 20× magnification. C. Fluorescence Spectrometry. Quantitative measurement of DiR fluorescence in the brain (AU per g of tissue) treated with IV LDL-DiR + FUS and IV LDL-DiR was determined by fluorescence spectrometry at λex and λem of 710 nm and 780 nm respectively (n=3). (**), (***) represent a significant difference from the corresponding groups at p<0.005 and p<0.001 respectively.