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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Biomaterials. 2016 Jan 7;83:194–206. doi: 10.1016/j.biomaterials.2016.01.013

Fig. 3. Expression of genes related to pluripotency in FReP cells during osteogenic differentiation in vitro.

Fig. 3

(a) NANOG, POU5F1, SOX2, and PODXL; (b) LEFTY1, DPPA4, and ZFP42; (c) GDF3; and (d) DNMT3β, SOX2 expression was analyzed with TaqMan® Gene Expression Assays (Life Technologies) and SsoFast™ Probes Supermix with ROX (Bio-Rad Laboratories)using three different cDNA templates obtained with iScript™ Reverse Transcription Supermix forqRT-PCR (Bio-Rad Laboratories). Expression of other genes was analyzed by qBiomaker™ Screening PCR Array (Qiagen) using three different cDNA templates. Concomitant GAPDH was used as a housekeeping standard. Data were normalized to un-reprogrammed BJ-fibroblasts (black dotted line). One-way ANOVA and Two-sample t-test were used to compare the data statistically (N = 3).