Fig. 3.

Wild chrysanthemum extract inhibited the upregulation of MMP-2 and MMP-9 induced by UVB radiation. a HaCat cells were irradiated by UVB (or preincubated with wild chrysanthemum extract) and processed for RNA isolation and semi-quantitative PCR. The cDNAs of MMP-2 and MMP-9 were amplified for 30 cycles and that of GAPDH for 20 cycles. b The MMP-2 and MMP-9 concentrations in the culture supernatant in (a) were determined with ELISA. The data in (b) represent the mean ± SD of triplicate samples; **(p < 0.01); *(p < 0.05); ns (p > 0.05) (Student’s t test). The data in (a) are representative of at least two independent experiments