Fig. 4.

Detection of pluripotent as well as neuronal markers via semi-quantitative PCR. Lane A refers to undifferentiated SHED; Lane B denotes gene expression using total RNA from Brain; Lane C shows gene expression of pre-conditioned SHED and Lane D shows gene expression of SHED without preconditioning. Pluripotent markers (POU5F1, SOX2, REX01, and ABCG2) were highly expressed in undifferentiated cells (Lane A) whereas reduced expression was observed in Total RNA from brain (Lane B), pre-conditioned SHED (Lane C) as well as in SHED without pre-conditioning (Lane D). Early neuronal markers (NES, NANOG, NR4A2, PAX6, and MSl1) were present in both Lane C and Lane D with the former showing an increased expression. Similarly, mature neuronal markers (GFAP, beta-Tub, TH, DAT, and NCAM) were shown to present in both Lane C and Lane D with the former showing an increased expression