Table 5.

Full experimental central composite design with coded and actual level of variables and the response function

Run	Type	A:Succinic acid (g/100 ml)		B:pH		C:Temperature (°C)		Siderophore unit  %
		Coded	Actual	Coded	Actual	Coded	Actual	Experimental	Predicted
1	Factorial	+1	0.52	−1	6	−1	22	44.67	50.02
2	Factorial	+1	0.52	+1	8	+1	34	48.87	52.49
3	Factorial	−1	0.28	+1	8	−1	22	29.58	32.26
4	Axial	+α	0.601815	0	7	0	28	70.97	61.89
5	Axial	0	0.4	−α	5.31821	0	28	26.46	25.42
6	Center	0	0.4	0	7	0	28	63.97	63.93
7	Factorial	0	0.52	+1	8	−1	22	46.86	51.32
8	Axial	−α	0.198185	0	7	0	28	23.76	25.89
9	Factorial	−1	0.28	−1	6	−1	22	25.84	25.49
10	Factorial	−1	0.28	+1	8	+1	34	36.28	34.21
11	Center	0	0.4	0	7	0	28	63.73	63.93
12	Axial	0	0.4	0	7	+α	38.0908	46.34	47.35
13	Factorial	−1	0.28	−1	6	+1	34	24.15	22.96
14	Axial	0	0.4	+α	8.68179	0	28	39.56	35.97
15	Center	0	0.4	0	7	0	28	63.27	63.93
16	Factorial	+1	0.52	−1	6	+1	34	46.13	46.72
17	Center	0	0.4	0	7	0	28	63.42	63.93
18	Center	0	0.4	0	7	0	28	64.32	63.93
19	Axial	0	0.4	0	7	−α	17.9092	54.13	48.49
20	Center	0	0.4	0	7	0	28	64.08	63.93