Table 1.
Different media used for the amidase production by G. subterraneus RL-2a
| Medium code | Composition (g/l) | Growth (mg dcw/ml) | Activity (U/mg dcw) | Reference | |
|---|---|---|---|---|---|
| M1 | Peptone | 5.0 g | 0.12 | 0.10 | Nutrient broth |
| Beef extract | 3.0 g | ||||
| M2 | Peptone | 12.5 g | 0.04 | 0.07 | Piotraschke et al. (1994) |
| Beef extract | 3.0 g | ||||
| Yeast extract | 5.0 g | ||||
| NaCl | 5.0 g | ||||
| M3 | Glucose | 15.0 g | 0.05 | 0.06 | Rao et al. (2008) |
| Peptone | 5.0 g | ||||
| Malt extract | 3.0 g | ||||
| Yeast extract | 3.0 g | ||||
| M4 | Glycerol | 10.0 g | 0.17 | 0.09 | Robas et al. (1993) |
| Peptone | 5.0 g | ||||
| Malt extract | 3.0 g | ||||
| Yeast extract | 3.0 g | ||||
| M5 | Glucose | 10.0 g | 0.09 | 0.21 | Scriven et al. (1998) |
| Na2HPO4·12H2O | 2.5 g | ||||
| K2HPO4 | 2.0 g | ||||
| MgSO4·7H2O | 0.5 g | ||||
| FeSO4·7H2O | 0.3 g | ||||
| CaCl2·2H2O | 0.6 g | ||||
| Yeast extract | 1.0 g | ||||
| M6 | (NH4)2HPO4 | 5.0 g | 0.09 | 0.20 | APY mineral salt medium |
| Peptone | 5.0 g | ||||
| Yeast extract | 3.0 g | ||||
| K2HPO4 | 5.0 g | ||||
| MgSO4·7H2O | 0.2 g | ||||
| FeSO4·7H2O | 0.02 g | ||||
| M7 | Glycerol | 10.0 g | 0.19 | 0.09 | Strobel and Sulivan (1999) |
| K2HPO4 | 0.5 g | ||||
| KH2PO4 | 0.5 g | ||||
| MgSO4·7H2O | 0.1 g | ||||
| Yeast extract | 1.0 g | ||||
| Peptone | 5.0 g | ||||
| M8 | Peptone | 20.0 g | 0.13 | 0.06 | Trott et al. (2002) |
| NaCl | 5.0 g | ||||
| Glucose | 2.0 g | ||||
| Yeast extract | 3.0 g | ||||
| Beef extract | 3.0 g | ||||
| M9 | Tryptone | 30.0 g | 0.14 | 0.13 | Vaidya et al. (2009) |
| Yeast extract | 15.0 g | ||||
| NaCl | 5.0 g | ||||
| Glucose | 2.0 g | ||||
| M10 | K2HPO4 | 2.0 g | 0.04 | 0.10 | Venkata Mohan et al. (2007) |
| NaCl | 1.0 g | ||||
| MgSO4 | 0.01 g | ||||
| FeSO4·7H20 | 0.02 g | ||||
| Biotin | 2 × 10−5 g | ||||
| Thiamine | 0.004 g | ||||
| Inositol | 0.002 g | ||||
| M11 | Peptone | 12.5 g | 0.11 | 0.09 | Self-formulated |
| Yeast extract | 3.0 g | ||||
| Beef extract | 5.0 g | ||||
| NaCl | 5.0 g | ||||
| FeSO4·7H20 | 0.01 g | ||||
| M12 | Glycerol | 10.0 g | 0.12 | 0.23 | Self-formulated |
| NaCl | 2.0 g | ||||
| K2HPO4 | 1.0 g | ||||
| MgSO4·7H2O | 0.5 g | ||||
| Beef extract | 0.2 g | ||||
| CaCl2 | 0.3 g | ||||