Table 3.
L18 (21 × 37) orthogonal array of designed experiments
| S. No. | 1 (pH) | 2 (Temp) | 3 (Sucrose) | 4 (K2HPO4) | 5 (NaCl) | 6 (yeast extract) | 7 (MgSO4) | 8 (CaCl2) | Amidasea activity (U/mg dcw) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0.14 |
| 2 | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 0.32 |
| 3 | 1 | 1 | 3 | 3 | 3 | 3 | 3 | 3 | 0.04 |
| 4 | 1 | 2 | 1 | 1 | 2 | 2 | 3 | 3 | 0.07 |
| 5 | 1 | 2 | 2 | 2 | 3 | 3 | 1 | 1 | 0.03 |
| 6 | 1 | 2 | 3 | 3 | 1 | 1 | 2 | 2 | 0.69 |
| 7 | 1 | 3 | 1 | 2 | 1 | 3 | 2 | 3 | 0.06 |
| 8 | 1 | 3 | 2 | 3 | 2 | 1 | 3 | 1 | 0.14 |
| 9 | 1 | 3 | 3 | 1 | 3 | 2 | 1 | 2 | 0.02 |
| 10 | 2 | 1 | 1 | 3 | 3 | 2 | 2 | 1 | 0.11 |
| 11 | 2 | 1 | 2 | 1 | 1 | 3 | 3 | 2 | 0.08 |
| 12 | 2 | 1 | 3 | 2 | 2 | 1 | 1 | 3 | 0.28 |
| 13 | 2 | 2 | 1 | 2 | 3 | 1 | 3 | 2 | 0.37 |
| 14 | 2 | 2 | 2 | 3 | 1 | 2 | 1 | 3 | 0.13 |
| 15 | 2 | 2 | 3 | 1 | 2 | 3 | 2 | 1 | 0.05 |
| 16 | 2 | 3 | 1 | 3 | 2 | 3 | 1 | 2 | 0.05 |
| 17 | 2 | 3 | 2 | 1 | 3 | 1 | 2 | 3 | 0.10 |
| 18 | 2 | 3 | 3 | 2 | 1 | 2 | 3 | 1 | 0.09 |
aValues indicate mean of duplicate observations