Table 2.
Histopathology grades of liver
| Eosinophilic alterationa | Nuclear dysmorphologyb | Fatty change | ||||
|---|---|---|---|---|---|---|
| Grade 0 | Grades 1, 2 | Grade 0 | Grades 1, 2 | Grade 0 | Grades 1, 2, 3, 4 | |
| Males | ||||||
| Control | 10 | 0 | 10 | 0 | 10 | 0 |
| PFOA | 14 | 6 | 13 | 7 | 16 | 4 |
| Eosinophilic alteration | Nuclear dysmorphologyb | Fatty change | ||||
|---|---|---|---|---|---|---|
| Grades 0, 1 | Grades 2, 3 | Grade 0 | Grades 1, 2 | Grade 0 | Grades 1, 2, 3 | |
| Females | ||||||
| Control | 7 | 3 | 9 | 0 | 10 | 0 |
| PFOA | 11 | 8 | 12 | 7 | 17 | 2 |
Grades were defined through a first blinded screening of sections and represent a range for focus of eosinophilic alteration (left) from no (grade 0), moderate (grade 1), to strong (grade 3); a range of nuclear dysmorphology, e.g., anisokaryosis/karyomegaly (middle) from no (grade 0), moderate (grade 1), to strong (grade 2) and a range of fatty change (right) from no (grade 0), moderate (grade 2), to strong (grade 4). In this distribution table, numbers are counts of perinatally control and PFOA-exposed (300 and 3000 µg PFOA/kg body weight/day) individual males (upper) and females (bottom) with a given grade. Results for both PFOA dose groups were similar, and data have been combined for statistical power
aThe distribution in the PFOA-exposed males versus control males is nearly statistically significant (p = 0.07) in a two-tailed Fisher’s exact test
bThe distribution in the PFOA-exposed animals versus control animals in both males and females is nearly statistically significant (for both p = 0.06) in a two-tailed Fisher’s exact test