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. 2016 Feb 12;7:10662. doi: 10.1038/ncomms10662

Figure 2. Lack of amino acids or serum alone is sufficient to relocalize TSC2 to lysosomes in different cell lines.

Figure 2

(a–d) Amino-acid or serum starvation causes lysosomal relocalization of TSC2 in breast adenocarcinoma MCF-7 cells. Treatments to assay the effects of amino-acid starvation and re-addition (a,b) or the effects of serum starvation and insulin re-stimulation (c,d) on TSC2 localization in MCF-7 cells were performed as in Fig. 1a,c, respectively (representative of three biological replicates). (e–h) Same as in a–d, using MEF NIH3T3 cells (representative of two biological replicates). For all panels, the degree of co-localization between TSC2 and LAMP2 (automatically thresholded MCC) is shown as mean±s.e.m. ***P<0.001, **P<0.01 comparing samples as indicated, using one-way ANOVA. See also Supplementary Figs 3–5. re-add., re-addition.