Table 1.
PCR conditions and primers used in this study.
| Target gene | Primer sequences | Amplicon size (bp) | References |
|---|---|---|---|
| mecA | 5′-TGGCTATCGTGTCACAATCG-3′ 5′-CTGGAACTTGTTGAGCAGAG-3′ |
310 | Vannuffel et al., 1995 |
| femA | 5′-CTTACTTACTGGCTGTACCTG-3′ 5′-ATGTCGCTTGTTATGTGC-3′ |
686 | Vannuffel et al., 1995 |
| Pvl (lukS-PV/lukF-PV) | 5′-ATCATTAGGTAAAATGTCTGGACATGATCCA-3′ 5′-GCATCAASTGTATTGGATAGCAAAAGC-3′ |
433 | Lina et al., 1999 |
| IS256 | 5′-AGTCCTTTTACGGTACAATG-3′ 5′-TGTGCGCATCAGAAATAACG-3′ |
762 | Gu et al., 2005 |
| Tst | 5′-ATGGCAGCATCAGCTTGATA-3′ 5′-TTTCCAATAACCACCCGTTT-3′ |
349 | Jarraud et al., 1999 |
The presence of genes was tested by amplification of the respective gene-fragments using 10 μl RedTaq® ReadyMix™ PCR Reaction Mix (Sigma, St Louis, MO), 1 μl gDNA, and 10 pmol/μl primer. Amplification conditions were as follows: an initial step of 5 min at 95°C, 40 cycles each of 30 s at 95°C, 45 s at 55°C, and 45 s at 72°C, and a final step of 45 s at 72°C.