Skip to main content
. 2016 Feb 16;10:5. doi: 10.3389/fncel.2016.00005

Table 2.

Fixation protocols used in Option B (perfused fixed tissue preparation).

graphic file with name fncel-10-00005-i0002.jpg

Optimal protocols for AIS components detection are highlighted with green. 4% PFA + acetone method was reproduced with four mice per group (Fgf14+∕+ and Fgf14−∕−), ≤ 3 sections per mouse. 1% Formaldehyde + 0.5% MeOH method was reproduced with five mice per group (Fgf14+∕+ and Fgf14−∕−), ≤ 3 sections per mouse. 4% PFA immersion method was reproduced with two mice, ≤ 4 sections per mouse. PFA, Paraformaldehyde, MeOH, Methanol, PBS, Phosphate-buffered solution, *Optimal fix™: Alcohol based tissue fixative that contains 50% Ethanol, 50% Ethanediol, 1% Zinc-chloride (MasterTech Scientific).