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. 2016 Feb 16;7:142. doi: 10.3389/fpls.2016.00142

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Copyright © 2016 Hofbauer, Melnik, Tschofen, Arcalis, Phan, Gresch, Lampel, Conrad and Stoger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Production and isolation of H5-zein and H5. (A) Immunoblot analysis of a leaf extract containing H5-zein. (B) Immunoblot of H5-zein samples following the sucrose density gradient separation of leaf homogenates. Lanes 1–5: Selected samples from different density steps along the gradient are shown (lane 1: sample from the top of the gradient; lane 5: pellet. 4G and 5G are the same fractions as 4 and 5, but obtained from a different experiment). An antibody specific for the His₆ tag was used for detection. (C) H5 after IMAC purification (3 μg were leaded on the gel and stained with Coomassie). wt, wild type; L, molecular weight ladder in kDa.