FIGURE 3.

Immunization timeline and IgG responses in the seven treatment groups. (A) All mice were injected with a primary dose of H5-zein (6 μg/ml) or H5 (0.6 mg/ml), both in sterile PBS, with or without Freund’s complete adjuvant. For the second and third injections, the adjuvant was switched to Freund’s incomplete adjuvant. (B) ELISA analysis of the anti-H5 IgG response following the third immunization. A single dot represents the ELISA result from a single serum sample. Each treatment group comprised 10 mice.