Table 1.
Effect of NO-PTMs on components of Asa-GSH cycle.
| Protein | NO-PTM | Effects | Target | Plant species | Reference |
|---|---|---|---|---|---|
| Ascorbate peroxidase (APX) | Tyrosine nitration | Decreased activity | Tyr235(1) | Pisum sativum | Begara-Morales et al., 2014 |
| S-nitrosylation | Increased activity | Cys32(1)(2)(3) | Arabidopsis thaliana, | Begara-Morales et al., 2014; | |
| Pisum sativum | Yang et al., 2015 | ||||
| Monodehydro-ascorbate reductase (MDAR) | Tyrosine nitration | Decreased activity | Tyr345(1) | Pisum sativum | Begara-Morales et al., 2015 |
| S-nitrosylation | Decreased activity | Cys68(3) | Pisum sativum | Begara-Morales et al., 2015 | |
| Dehydro-ascorbate reductase (DHAR) | Tyrosine nitration | N.D. | N.D. | N.D. | Fares et al., 2011; Kato et al., 2013; Puyaubert et al., 2014 |
| S-nitrosylation | Decreased activity | Cys20(1)(2), | Arabidopsis thaliana; | ||
| Cys147(1)(2) | Solanum tuberosum | ||||
| Glutathione reductase (GR) | Tyrosine nitration | No effect | N.D. | Pisum sativum | Begara-Morales et al., 2015 |
| S-nitrosylation | |||||
Nitration and S-nitrosylation targets have been identified by different technological approaches: (1) Mass spectrometry, (2) site-directed mutagenesis, and (3) in silico identification. ND: Not determined.