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. 2015 Oct 13;24(1):156–165. doi: 10.1038/mt.2015.172

Figure 2.

Figure 2

Activation of cAMP signaling inhibits M1 virus-induced antiviral factor expression. (a–f) The colorectal cancer cell line HCT-116 was infected with M1 virus (10 plaque forming unit (PFU)/cell) and interferon cascade genes were quantified. IFNA (a), IFNB (b), IRF3 (c), IRF7 (d), MDA5 (e), and IFIT1 (f) show mRNA fold-expression at 0, 6, 12, and 24 hours after M1 infection (mean ± SD). (g–l) HCT-116 cells were infected with M1 virus (10 PFU/cell) in the presence or absence of db-cAMP (1 mmol/l) or Forskolin (10 μmol/l), and mRNA levels were quantified by reverse transcription-polymerase chain reaction (mean ± SD). IFNA (g), IFNB (h), IRF3 (i), IRF7 (j), MDA5 (k), and IFIT1 (l) show mRNA fold-expression after M1 infection and db-cAMP treatments. Fold-expression of genes was normalized to β-actin. *P < 0.05; **P < 0.01.