Figure 7.

8-CPT-cAMP enhances M1 oncolysis in primary tumor specimens. Surgical liver cancer specimens 50–100 mg were cut into small pieces (1 mm³) and cultured in Dulbecco's modified Eagle's medium containing 15% fetal bovine serum at 37 °C. Tissues were then treated with vehicle (OPTI-SFM), 5-fluorouracil (5-Fu, 100 μl/ml), M1 (5 × 10⁷ plaque forming unit (PFU)), 8-CPT-cAMP (1 mmol/l), M1 plus 8-CPT-cAMP, or HgCl₂. Tissue viability was assessed by tissue culture-end point staining-computer image analysis (TECIA) after MTT staining. (a) Representative images of ex vivo tumor specimens. The left panel indicates the area of the tissue and the right panel indicates living cells after drug treatment (MTT staining) captured by the TECIA system. (b) Inhibition percentages corresponding to the calculated areas displayed in a. (c) Cytotoxicity after treatment of four specimens (*, not detectable).