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Stability of formulated lipidoid nanoparticles tested in vitro on the day of synthesis (day 0) and up to 15 days in storage at 4 °C. Geometric mean fluorescence intensity for cultured HeLa cells was measured 24 hours after transfection with 40 ng eGFPmodRNA (gray), relative to nontreated cells (mean ± SD, n = 3). Particle size (z-average diameter) was determined by dynamic light scattering (black) (mean ± SD, n = 3).
