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. 2015 Dec 8;24(1):135–145. doi: 10.1038/mt.2015.201

Figure 5.

Figure 5

The double-stranded stem region of IVT-B2 is essential for selective cytotoxicity to cancer cells. (a) PC3 and PNT2 cell survival rates were detected 48 hours after IVT-RNAs (1.16 ng/ml) transfection with MTS assay. (b) IVT-RNAs was transfected into RIG-I-knocked-down PC3 cells (RIG-I siRNA-transfected) or control PC3 cells (scramble siRNA-transfected). IFN-β secretion was assessed 48 hours after IVT-B2 RNA transfection. “Lipo” indicates cells that were treated with Lipofectamine RNAiMAX without RNA. (c) PC3 cell survival rates were detected 48 hours after IVT-RNAs transfection with MTS assay. The mean ± SD is shown (n = 3). *P < 0.05; **P < 0.01. (n = 3). (d) Expression levels of RIG-I, MAVS, TRAIL, and Noxa were analyzed by western blot 24 hours after IVT-RNAs transfection into PC3 cells. MTS, 3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt.