Figure 1.

BIA biosynthesis pathway representing the manipulated genes and several analyzed transcripts after VIGS and overexpression assays. Both 4′OMT [(S)-3′-hydroxy- N-methylcoclaurine 4′-O-methyltransferase] and 7OMT (reticuline 7-O-methyltransferase) were manipulated. Transcripts that analyzed via qRT-PCR were shown in red. Long arrows point out more than one reaxion. Abbreviations: TyrAT, tyrosine aminotransferase; TYDC, tyrosine decarboxylase; NCS, norcoclaurine synthase; 6-OMT, (S)-norcoclaurine 6-O-methyltransferase; CNMT, (S)-coclaurine N-methyltransferase; NMCH, (S)-N-methylcoclaurine-3′-hydroxylase; SalSyn, salutaridine synthase; SalR, salutaridine reductase; SAT, 7(S)- salutaridinol 7-O-acetyltransferase; CODM, codeine O-demethylase; T6ODM, thebaine 6-O-demethylase; COR, codeinone reductase; SOMT, scoulerine 9-O-methyltransferase; CAS, (S)-canadine synthase; TNMT; tetrahydroprotoberberine N-methyltransferase; CYP82Y1, N-methylcanadine 1-hydroxylase; NOS, noscapine synthase.