Figure 6. Kv11.1-1A and Kv11.1-3.1 show different channel ubiquitinylated changes in response to bortezomib.

HA tagged hERG channels were immunoprecipated from stably expressing hERG cell lysates that had been incubated with bortezomib or DMSO for 16 hours. Cell lysates and IP elutions were probed for HA tag, NaK ATPase, and ubiquitin. Cell lysates show increases in total ubiquitin with bortezomib treatment and both 1A and 3.1 channels show poly-ubiquitinylation. Immunoprecipitated 1A channels show increase in ubiquitnylation accumulation in bortezomib treatments. Immunoprecipitated 3.1 channels from bortezomib treated cells show less accumulation than DMSO incubated cells.