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. 2016 Feb 16;6:18823. doi: 10.1038/srep18823

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Increased exposure time to X-ray irradiation or heat (65 °C) resulted in decreased reproductive viability (A,B) and metabolic activity (C,D) of ATCC 27853 cells. Bacterial viability was determined by counting CFUs on agar plates, and metabolic activity was measured by monitoring the OD using Alamar Blue colorimetric assay. X ray irradiation diminished ATCC 27853 reproductive viability but does not impair its metabolic activity. The arrow denotes the irradiation dose, which could reduce the reproductive viability to 0% while retained 63.33% ± 4.49% metabolic activity compared with the live cells (C). Results represent three independent experiments and are expressed as mean ± SD (n = 5).