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. 2016 Feb 16;6:18823. doi: 10.1038/srep18823

Figure 7. Nasal immunization with irradiated vaccine elicits Th17 cells responses.

Figure 7

(A) IL-17 production by splenic T cells isolated from nasal immunized mice and then stimulated for 24 h and 48 h in the presence of heat killed whole bacterial cells of PA strain PAO-6 along with irradiated splenocytes. Immunized T cell secreted higher IL-17 than the controls, and IL-17 secretion decreases to baseline when splenic T cells are co-cultured with anti-CD4 monoclonal antibody (clone GK1.5, 1μg/well) at both time points (n = 3, ANOVA, *p < 0.05); (B) At 6h and 18h after PAO-6 challenge, spleen cells were recovered, gated by CD4 and CD8 for lymphocytes identification, and immunized mice has significantly higher CD4+ T cells compared with controls. FACS data are presented as means ± SD and represent three independent experiments (n = 5, ANOVA, *p < 0.05); (C) At 6 h and 18 h after PAO-6 challenge, spleen cells were recovered, gated by CD4 and IL-17 for lymphocytes identification, and immunized mice has significantly higher CD4+ IL-17+ Th17 cells compared with controls. FACS data are presented as means ± SD and represent three independent experiments (n = 5, ANOVA, *p < 0.05).