Figure 5. Imaging of Mlp37 with a fluorescently labelled ligand.
(a) Competitive labelling of Mlp37 with carboxyfluorescein-labelled l-serine [Ser-FAM]. Vmlp201 (∆mlp24 ∆mlp37) cells carrying the vector (none) or pMlp37 (Mlp37) were incubated in TMN buffer containing 100 μM Ser-FAM supplemented with none (+none), 1 mM serine (+Ser), 1 mM taurine (+taurine), or 1 mM glutamate (+Glu). (b) The fraction of cells with polar Ser-FAM spots in the absence or presence of an attractant. (c) Co-localisation of Ser-FAM with Mlp37. Vmlp201 cells expressing Mlp37-TagRFP were treated with Ser-FAM: left, Mlp37-TagRFP; middle, Ser-FAM; right, merge.