Table 1.
Primary antibodies used in this study.
| Antigen | Immunogen | Manufacturer, host, monoclonal vs. polyclonal | Catalog # | RRID | Dilution used |
|---|---|---|---|---|---|
| Histamine | Synthetic HA coupled to succinylated keyhole limpet Hemocyanin with carbodiimide linker | Immunostar, Rabbit, polyclonal | 22939 | AB_572245 | 1:500 |
| Bruchpilot | Bruchpilot peptide sequence (1390-1740) from head homogenate | DSHB, Mouse, monoclonal | Nc-82 | AB_2314866 | 1:50 |
| Manduca sexta HA B receptor (MsHisClB) | Histamine B receptor peptide sequence (VNPDIELPQLD) | Bethyl Laboratory (custom), Rabbit, polyclonal | N/A | N/A | 1:5000 |
| γ-aminobutyric acid (GABA) | Purified GABA conjugated to BSA | Abcam, Mouse, monoclonal | ab49675 | AB_880138 | 1:500 |
| Allatotropin | Allatotropin coupled to thyroglobulin with glutaraldehyde | Dr. Jan Veenstra, Rabbit, polyclonal | N/A | AB_2313973 | 1:8∗ |
| FMRF-amide | Synthetic FMRF coupled to bovine thyroglobulin with gluteraldehyde | Dr. Eve Marder, Rabbit, Polyclonal | N/A | AB_572232 | 1:8∗ |
∗See fluorescent tagging subsection of the methods for details.