Table 2. r₀ values for GFP-RFP FRET pairs.

FRET pair	φDa	εAb	r0c (nm)
mEGFP-mCherry	0.60	72	5.4d
Clover-mCherry	0.76	72	5.8d
Clover-mRuby2	0.76	113	6.3d
mEGFP-mRuby3	0.60	128	5.8
Clover-mRuby3	0.76	128	6.5
mClover3-mRuby3	0.78	128	6.5
mNeonGreen-mRuby3	0.80	128	6.5

^aQuantum yield of donor.

^bExtinction coefficient of acceptor in mM⁻¹ cm⁻¹.

^cCalculated Förster radius assuming random interfluorophore orientation (κ² = 2/3).

^dData from ref. 1.