Table 3. Evolution of mClover3.

Clover variant	εa	φb	Brightnessc	Stoichiometryd	Photostabilitye
Clover	111	0.76	84	monomer	50
Clover N149Y (Clover1.5)	118	0.78	92	monomer-dimer	66
Clover1.5 A206K (mClover1.5)	109	0.79	86	monomer	66
Clover1.5 G160S (dClover2)	123	0.80	98	dimer	98
dClover2 A206K	117	0.78	91	dimer	95
dClover2 A206K S160C (mClover3)	109	0.78	85	monomer	80

^aExtinction coefficient at peak in units of mM⁻¹ cm⁻¹.

^bTotal quantum yield.

^cProduct of ε and φ.

^dBy size-exclusion chromatography at a loading concentration of 10 μM.

^eTime in s to photobleach from 1,000 to 500 photons per s per molecule under arc-lamp illumination.