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. 2016 Feb 16;6:21206. doi: 10.1038/srep21206

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Copyright © 2016, Macmillan Publishers Limited

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(A) DAPI-stained E13.5 wt mouse telencephalon; single 1.5-μm optical section. Box: region of dorsolateral telencephalon examined in this study; V, ventricle. Scale bar, 100 μm. (B) Cartoon illustrating major features of aRG morphology and INM. (C) aRG in dorsolateral telencephalon of E13.5 GFAP::GFP transgenic mouse embryo, identified by GFP immunofluorescence (green, stack of 30 0.8-μm optical sections) combined with DAPI staining (blue, single 0.8-μm optical section at z-position of the indicated nuclei); arrowheads: apical process contacting ventricle (bottom) and basal process reaching the basal lamina (top). Scale bar, 20 μm. IZ, intermediate zone; in (C-F), the CP and IZ are indicated together; in the following figures, these layers are indicated as CP only. (D) aRG nuclei in VZ of dorsolateral telencephalon of E13.5 wt mouse embryo immunostained for Pax6 (green) combined with DAPI staining (blue, 0.8-μm optical sections). Scale bar, 10 μm. (E) Golgi apparatus in dorsolateral telencephalon of E14.5 wt mouse embryo revealed by GRASP-65 (red) and giantin (green) double immunofluorescence combined with DAPI staining (blue, 0.8-μm optical sections). Scale bar, 20 μm. (F) Subcellular localization of Golgi apparatus, revealed by GRASP-65 immunofluorescence (red), in an individual aRG in dorsolateral telencephalon of E13.5 GFAP::GFP transgenic mouse embryo, identified by GFP immunofluorescence (green, stack of 13 0.8-μm optical sections). Diagram: localization of all Golgi units (arrows) in the cell as reconstructed from the series of optical sections. Dashed lines, ventricular surface (bottom) and basal lamina (top). Scale bar, 10 μm. (G) Bottom row: presence of Golgi apparatus in the apical process of the aRG shown in (F). Top row: absence of Golgi apparatus in the basal process of another aRG, analyzed as in (F); dashed lines, basal lamina. Scale bars, 2 μm. (H,I) Z-stacks of APs in VZ of dorsolateral telencephalon of E14.5 wt mouse embryo co-electroporated in utero at E13.5 with plasmids encoding mCherry and either GalNAcT2-GFP (H) or GM130-GFP (I). Images are consecutive 0.8 μm optical sections. Diagrams: localization of all Golgi units (arrows) in the respective APs. Dashed lines, ventricular surface. Scale bars, 10 μm.