Figure 2. Hexsoamine biosynthetic pathway involved in the maintenance of CSC-like phenotype in PLC8024 cell line.

(A) AZA treatment reduces the percentage of CD133 in PLC8024 parental cells. (B) Expression of CD133 was determined by western blotting. The PLC8024 was cultured in high glucose medium in presence of AZA with different concentrations (5, 10, 25, 50, 100 μM ) or a vehicle control (DMEM only). Beta-actin was used as loading control.