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. 2016 Feb 16;6:21184. doi: 10.1038/srep21184

Figure 6. The supplementation of GlcNAc plays an important role in cancer cell survival under low glucose condition.

Figure 6

(A) PLC8024 cells were seeded in 6-well plate and maintained in conditional medium with indicated glucose concentration (25 mM, 2.5 mM, 0.75 mM and 0 mM) in presence of 10 mM GlcNAc or the vehicle control (DMEM only) for 48 h. The representative images were captured by bright field microscope. (B) The histogram represents quantitative analysis of the mean values of survival cells in triplicate determinations. Asterisk indicates the significant difference. *P  0.05. (C) The conditional soft agar assay medium with different glucose concentration (25 mM, 10 mM, 5 mM, 2.5 mM) were pre-prepared. The CD133-positive subpopulation cells and CD133-negative counterpart isolated from PLC8024 were maintained in conditional soft agar assay medium with additional of 10 mM GlcNAc or the vehicle control (DMEM only) for 3 weeks. The representative images were captured by bright field microscope. All pictures were all at same magnification, resolution and size. The histogram represents the quantitative analysis of mean values of colony formation in conditional soft agar assay medium in triplicate determinations. Asterisk indicates the significant difference. *P  0.05.