Skip to main content
. Author manuscript; available in PMC: 2016 Feb 16.
Published in final edited form as: Cell. 2008 May 2;133(3):427–439. doi: 10.1016/j.cell.2008.03.020

Figure 5. Visualization of Microtubule Binding.

Figure 5

(A) A mix of rhodamine-labeled tubulin:tubulin at a 0.16 ratio and final concentration of 100 nM was polymerized and incubated with increasing amounts of Alexa-Ndc80bonsai. N/T is the Ndc80bonsai/tubulin concentration ratio.

(B) Inset from N/T = 0.125 showing clusters of fluorescent Ndc80bonsai on microtubules.

(C) Line scans of two representative microtubules. The net fluorescence intensity (signal – background) at each pixel was divided by the mean net fluorescence intensity along the line scan.

(D) Inset from N/T = 4.0 showing even distribution of Ndc80bonsai.

(E) Line scans of two representative microtubule analyses as for (C).