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. 2016 Feb 16;6:21133. doi: 10.1038/srep21133

Figure 4. Nrf2 mediates FST induction during SiO2 NP treatment.

Figure 4

(A–C) A549 cells transfected with siRNA targeting Nrf2 (siNrf2) or control siRNA were incubated with or without 50 μg/ml of SiO2 NPs for another 12 h. The mRNA level of Nrf2 (A) and FST (B) was detected by qPCR and the protein level was detected by immunoblotting (C,D) Schematic of a luciferase reporter of the human FST promoter containing an evolutionarily conserved ARE among vertebrate species. (E) A549 cells transfected with siRNA targeting Nrf2 or control siRNA together with wild-type or ARE-deleted FST promoter luciferase reporter construct. Luciferase activity was measured 24 h following plasmid transfection. (F) A549 cells were treated with or without 50 μg/ml of SiO2 NPs for 12 h. ChIP analysis was performed with antibodies against Nrf2 and analyzed by qPCR. The values are the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 (One way ANOVO).