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. 2016 Feb 16;16:108. doi: 10.1186/s12885-016-2146-z

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© Lima et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Overexpression of miR-146b-5p enhances cell spreading and formation of large lamellipodia in non tumor rat thyroid follicular cell line (PCCl3). Cells were seeded upon glass coverslips (18 mm) without and with Matrigel® coating (10 μg/ml) and cultured for 24 h. After this period, cells were fixed, stained for F-actin (green) and nucleus (blue) and analyzed by confocal microscopy. PCCl3-WT: non tumor rat thyroid follicular cell line, PC-CMV-ø: cell transfected with an empty vector, PC-CMV-146b: cell transfected with the miR-146-5p genomic region. Bars: 10 μm