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. 2016 Feb 16;16:108. doi: 10.1186/s12885-016-2146-z

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© Lima et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Overexpression of SMAD4 decreases migration and invasion of BCPAP cells. Cells were transfected with pBabe-puro-Smad4-Flag and pBABE-puro plasmids (control) as described in the Methods section. a The SMAD4 gene expression was analyzed by qPCR. RPL19 gene expression was used for normalization. b, c Transwell migration (without basement membrane) and invasion (with basement membrane) assays were performed during 8 h. pBabe ø: BCPAP transfected with pBABE-puro plasmids; pBabe SMAD4: BCPAP transfected with pBabe-puro-Smad4-Flag. d For SMAD4 protein expression, 50 μg of total protein per sample were used. α -Tubulin expression was used for normalization. Statistically significant differences: * P< 0,01 (pBabe SMAD4 versus pBabe ø)