Fig. 5.

Changes of breath hydrogen excretion after ingestion of RG or HRG in healthy human subjects. FOS, fructooligosaccharide; RG, resistant glucan; HRG, hydrogenated resistant glucan. Test solution containing 30 g of purified RG or HRG was ingested by 10 healthy subjects shown in Fig. 4 after overnight fasting, and end-respiratory gas (750 ml) was collected using collection bag at 1-h intervals for 14 h after ingestion (a). Furthermore, to evaluate the available energy test solution containing 5 g of purified RG or HRG was ingested by healthy subjects after overnight fasting (b). FOS (5 g) was used as a typical oligosaccharide which is nondigestible and completely fermentable. Figure 5-a shows the change of hydrogen excretion 14 h after ingestion of 30 g of test substance, and Fig. 5-b shows the change of hydrogen excretion 14 h after ingestion of 5 g of test substance. Values were means ± SD. *, The excretion of breath hydrogen in RG or HRG ingestion were significantly lower versus that in FOS ingestion, at P < 0.05 by Steel method. There was no significantly difference between RG and HRG by Steel-Dwass method