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. 2016 Feb 16;6:21563. doi: 10.1038/srep21563

Figure 4. Maturation of primary rat OPCs using distinct substrates and conditions.

Figure 4

(A) Representative immunofluorescence microscopy images of primary rat oligodendrocytes stained for PLP (in green, and nuclei were counterstained with DAPI, in blue), cultured for 2 days in proliferation medium (2d PM), or for 3 or 5 days in differentiation medium (3d DM or 5d DM, respectively). Cells were maintained on TCPs or 6.5 kPa PAHs coated/functionalised with PDL or PDLMN, as indicated. Scale bars correspond to 50 μm. (B) The percentage of PLP-positive cells was quantified for each experimental condition (3 or 5 days in DM) and platform. Data represent the mean ± SEM of at least three independent experiments. Statistical analysis was performed by two-way ANOVA followed by Bonferroni post-test. (C) Quantification of CTCF for PLP signal of primary rat oligodendrocytes cultured with DM for 3 or 5 days on TCPs or 6.5 kPa PAHs coated/functionalised with PDL or PDLMN. Data represent mean ± SEM of at least 3 independent experiments. (D,F) Measurement of the PLP signal area of primary rat oligodendrocytes cultured with DM for 3 days (D) or 5 days (F) on TCPs or PAHs coated/functionalised with PDL or PDLMN. Data represent mean ± SEM of at least 3 independent experiments. Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparison test. (E,G) Mean fluorescence intensity (MFI) of PLP signal of oligodendrocytes cultured on TCPs or PAHs for 3 days (E) or for 5 days (G). Data represent mean ± SEM of CTCF of at least 3 independent experiments. Statistical analysis was performed by t-test. All statistical analysis was performed using the software GraphPad Prism 6 and the statistical significant differences were represented using the connectors (n.s.: non-significant, *p < 0.05, **p < 0.01, ***p < 0.001).