Fig. 1.

Synaptic pruning is disrupted in Pit1^dw IHCs. (a and b) Projection of confocal sections obtained from the mid-turn of cochlear whole mounts stained with afferent presynaptic (RIBEYE; green) and postsynaptic (SHANK1; red) markers at P5 in WT mice (a) and Pit1^dw mice (b). (c and d) The same markers at P14 in WT mice (c) and Pit1^dw mice (d). (e–i) Box plots of the quantification of RIBEYE, SHANK1 and RIBEYE–SHANK1 puncta from the mid-turn of the cochlea in WT and Pit1^dw mice. (e and f) RIBEYE, SHANK1 and RIBEYE–SHANK1 counts at P5, P9 and P14 in WT mice (e) and Pit1^dw mice (f). (g–i) Comparison of RIBEYE (g), SHANK1 (h) and RIBEYE–SHANK1 (i) counts between WT and Pit1^dw mice. significant comparisons (P < 0.05) are indicated with asterisks. The dots indicate outliers in the data.