Table 1.

Sequence of single guide RNA (gRNA), forward and reverse PCR primers (PCR-fw and PCR-rev), and primers used for sequencing (p1 and p2)

Gene	Type/Name	Sequence	Genomic location (Assembly Dec. 2013 GRCh38/hg38)
IRF3 encoded on (−) strand of genome	gRNA-1	GCCACTGGTGCATATGTTCC	19:49663483–49663502 (−)
	gRNA-2	CCACTGGTGCATATGTTCCC	19:49663482–49663501 (−)
	gRNA-3	ATAAGCCAGACCTGCCAACC	19:49663455–49663474 (−)
	PCR-fw	TTCTCACCTGGGTATCAGAAGTA	19:49663181–49663203 (+)
	PCR-rev	TGAGGTTCCTAACTACCGAATTA	19:49664294–49664316 (−)
	p1	TGTCTGGCTGGGAAAAGTC	19:49663232–49663250 (+)
	p2	CTGTAATCCCAGCACTTTG	19:49663960–49663978 (−)
TLR3 encoded on (+) strand of genome	gRNA-1	ACATTAGATCTGTCTCATAA	4:186078849–186078868 (+)
	gRNA-2	ATTAGGAACTCAGGTTCAGC	4:186078887–186078906 (+)
	gRNA-3	GGCTTGTCATCTACAAAATT	4:186078870–186078889 (+)
	PCR-fw	TGTGTTTGATAAGCCATGTGA	4:186078463–186078483 (+)
	PCR-rev	GGAGGCTAGAGAGGGAGAAC	4:186079465–186079484 (−)
	p1	AATCCTTCCTACAATGG	4:186078618–186078634 (+)
	p2	AGGATTGCTGGAAGACAGG	4:186079129–186079147 (−)
p65 encoded on (−) strand of genome	gRNA-1	TCAATGGCTACACAGGACCA	11:65661815–65661834 (−)
	gRNA-2	AGGGACAGTGCGCATCTCCC	11:65661796–65661815 (−)
	gRNA-3	AGCTTGTAGGAAAGGACTGC	11:65661737–65661756 (−)
	PCR-fw	AATGGTTTTCTTCCTCAAACAA	11:65661392–65661413 (+)
	PCR-rev	CTTAGTTTCACCGCAGGTTCTA	11:65662331–65662352 (−)
	p1	GTATCCCCTGGAACTCATC	11:65661487–65661505 (+)
	p2	TGTTCCCCCTCATCTTC	11:65662186–65662202 (−)
gfp	gRNA	AAGGGCGAGGAGCTGTTCAC	-