Fig. 1.

shRNA-mediated knockdown of oncogenic Kras reduces HK2 expression and suppresses growth in NSCLC cell lines. a, b Protein level of Kras and HK2 was detected in KP2 and H23 cells expressing shRNAs for Kras. c, d Clonogenic survival assays were performed to assess cell growth. Colonies were stained by crystal violet after 7 days of cell growth. e, f Xenograft tumor growth. 1 × 10⁶ cells expressing scramble shRNA (CON) or shRNA for Kras were subcutaneously injected to the lower flank of NSG mice. Representative images of tumors at 4 weeks after injection are shown (e). Quantification of tumors weight (P < 0.01) (f). g IHC staining of cell proliferation marker Ki67 from control or Kras-knockdown KP2 cells. h Quantification of Ki67 expression from representative images shown in g (P < 0.01)