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. 2015 Mar 21;96(4):887–900. doi: 10.1111/mmi.12979

Figure 1.

figure

Effective concentration 50% (EC 50) values for four trypanocidal drugs as determined using the Alamar Blue fluorescence assay. The strains tested were all derived from T . b. brucei s427 wild‐type (WT); B48 is a multidrug‐resistant strain lacking the genes for the TbAT1 and TbAQP2 drug transporters (Munday et al., 2014). B48 + EV is the resistant control of B48 transfected with the empty vector (EV) pHD1336 (Biebinger et al., 1997). All the variants of TbAT1 including the wild‐type copy (B48 + TbAT1) were expressed in B48 using this vector (Munday et al., 2013). Mutations (Mut) 1–6 are as listed in the box; Δ(F316) is the TbAT 1 allele from which the codon for Phe316 was deleted. The dotted line running across each panel denotes the EC50 value of the B48 + EV control for each drug. Statistical significance was determined using a one‐way ANOVA relative to B48 + EV, in GraphPad Prism 5.0. **, P < 0.01; ***, P < 0.001. Data shown are the average and SEM of at least five independent determinations.