Figure 2.
Transport of (A) 0.1 μM [3 H]‐adenosine or (B) 0.1 μM [3 H]‐diminazene aceturate by T . b. brucei B48 transfected with empty vector pHD1336 (B48 + EV) expressing either the wild‐type TbAT1 allele or a mutant TbAT1 allele as listed in Fig. 1. Incubation times were 30 s or 60 s for adenosine or diminazene transport respectively. All bars are the average of three independent experiments and error bars represent SEM; statistical significance was calculated relative to TbAT1WT (unless specifically indicated) using a paired Student's t‐test: *, P < 0.05; **, P < 0.02; ***, P < 0.01.